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Product Info Summary
| SKU: | EK0511-RB |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rabbit |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and rabbit milk. |
Product info
Product Name
Rabbit TGF Alpha ELISA Kit PicoKine®
SKU/Catalog Number
EK0511-RB
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rabbit TGF Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Rabbit TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and rabbit milk. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rabbit TGF Alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0511-RB)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V40-A89
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0511-RB for more details
Cross-reactivity
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Reactive Species
EK0511-RB is reactive to TGFA in Rabbit samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and rabbit milk.
Application Guarantee
EK0511-RB is guaranteed for ELISA in Rabbit by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGF-alpha
Transforming growth factor alpha (TGF-alpha) is upregulated in some rabbit cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to rabbit chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic rabbit TGF-alpha showed similar immunoreactivity when compared with rabbit TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0511-RB-CAP | Anti-Rabbit TGFA Pre-coated 96-well strip microplate | 1 |
| EK0511-RB-ST | Rabbit TGFA Standard | 2 vials, 10 ng/tube |
| EK0511-RB-DA | Rabbit TGFA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.102 | 0.199 | 0.291 | 0.443 | 0.711 | 1.156 | 1.676 | 2.315 |
Data Example Images
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Rabbit TGF alpha PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rabbit TGF Alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 118 | 225 | 516 | 126 | 238 | 509 |
| Standard deviation | 5.43 | 12.83 | 16.5 | 8.95 | 14.28 | 27 |
| CV (%) | 4.6 | 5.7 | 3.2 | 7.1 | 6 | 5.3 |
Protein Target Info & Infographic
Gene/Protein Information For TGFA (Source: Uniprot.Org, NCBI)
Gene Name
TGFA
Full Name
Protransforming growth factor alpha
Weight
17.006kDa
Alternative Names
protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFA, check out the TGFA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rabbit TGF Alpha ELISA Kit PicoKine® (EK0511-RB)
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No publications found for EK0511-RB
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Rabbit TGF Alpha ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Rabbit TGF-alpha Picokine® ELISA Kit (Catalog # EK0511-RB)?
Verified Customer
Verified customer
Asked: 2020-08-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TGF-alpha causing disruption of its protein structure. TGF-alpha may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TGF-alpha ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-11
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Campbell
Verified customer
Asked: 2019-09-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-09-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-05-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-05-12
Question
Q: What is the optimal O.D. value for TGF-alpha ELISA kit? I used your TGF-alpha ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGF-alpha even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGF-alpha ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGF-alpha that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2018-12-12
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TGF-alpha in Rabbit rabbit milk? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
J. Miller
Verified customer
Asked: 2015-11-23
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGF-alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2015-11-23
Question
Q: Can TGF-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2015-07-12
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2015-07-12
